Mavra Manzar

3 Questions 13 Answers 0 Followers

Questions related from Mavra Manzar

How to find out if a gene regulate's the transcription of other genes?

Hello all, I have transfected my cells with plasmid containing my shRNA sequences of my gene of interest and I'm looking this effect on 3 other genes, that when my gene is knockdown what happens...

09 January 2024 1,634 6 View

How to find out if a gene regulate's the transcription of other genes?

Hello all, I have transfected my cells with plasmid containing my shRNA sequences of my gene of interest and I'm looking this effect on 3 other genes, that when my gene is knockdown what happens...

09 January 2024 2,982 6 View

I am having ligation and transformation issues with cloning my shRNA into the vector?

I use restriction enzyme cloning method, and I have been using new reagents e.g., competent cells, and ligase reagent because initially I thought the problem is that these reagents were outdated....

13 October 2023 9,682 10 View