Maurizio Forte

3 Questions 10 Answers 0 Followers

Questions related from Maurizio Forte

Who can suggest a good protocol of IHC for detect beta amyloid in paraffin sections of rat brains?

There are other widely used marker for Alzheimer disease? I need to perform a colocalization? Fluorescent secondary antibodies or DAB (brains were fixed in Carnoy)? Nuclei staining? Any help on...

06 June 2016 1,875 1 View

How it is possible to explain why LNCaP cells did not migrate in my migration experiments using transwell?

I seeded LNCaP cells in transwell (medium: upper 1% FBS; lower 10% FBS). I found only few cells in the lower side of transwell after 48 hours. I performed the test with other cell lines...

12 December 2015 5,051 2 View

Does anyone have a good protocol or manual for culturing primary cells?

I have only worked with immortalized and tumoral cells. Now I have to separate epithelial cells from human tissues.

05 May 2014 4,645 5 View