Matthew Whitley

3 Questions 12 Answers 0 Followers

Questions related from Matthew Whitley

Problems with chitin-binding domain fusion protein NOT sticking to chitin resin?

Using the New England Biolabs IMPACT system, we have expressed our protein of interest with a C-terminal fusion of a chitin binding domain (vector is pTXB1 from the NEB IMPACT system). The...

10 October 2019 9,424 3 View

How to remove artifacts in digital camera images of SDS-PAGE gels?

In my work I have need to take pictures of objects sitting on a white light box (not a UV light box). When I try to do so with my Android phone's digital camera, these strange orange lines...

08 August 2018 8,538 2 View

Are there useful purification tags for denaturing purification conditions?

Hello all, I am working with a heterodimeric protein complex that I must form by expressing and purifying each component under denaturing conditions and then co-refolding the two denatured...

11 November 2014 4,570 3 View