Matthew Stabler

3 Questions 2 Answers 0 Followers

Questions related from Matthew Stabler

Could somebody help with long term shelf life times of coat proteins at various temps?

Could somebody please let me know the long term shelf life of coat proteins stored at -20* compared to -80*? Also, if a coat protein is previously frozen and then thawed, how long can it stay...

02 February 2017 1,609 0 View

11 year old glycerol stock of transformed cells not growing on media?

I have an 11 year old glycerol stock of a coat protein that is in pET21(d)+ expression vector and transformed with BL21-(DE3) transformation cells that will not grow on my LB agar w /ampicillin...

12 December 2016 8,546 2 View

After extracting the DNA from bacteria into a DNA extraction plate what would be the best way to safely dry the DNA to the wells of the plate?

I was thinking either ethanol or isopropyl alcohol precipitation would work but I can't seem to locate a good protocol for doing this. I need the wells to be dry because the plates I'm working...

01 January 1970 5,522 4 View