Matthew Herrera

4 Questions 1 Answers 0 Followers

Questions related from Matthew Herrera

Can someone help me with my immunohistochemistry protocol?

Hi, I am currently attempting to optimize an IHC protocol for some lymphatic endothelial cells that I'm studying. I've been having a lot of trouble getting consistently good images after...

11 November 2019 7,737 1 View

Do I need a loading control if I'm measuring phospho and total protein expression?

I am looking to measure phospho-Erk and total-erk protein expression with western blotting, and am wondering if it is necessary to use a loading control. I normally measure phospho first, then...

12 June 2019 8,319 2 View

Do I need a loading control if I'm measuring phospho and total protein expression?

I am looking to measure phospho-Erk and total-erk protein expression with western blotting, and am wondering if it is necessary to use a loading control. I normally measure phospho first, then...

06 June 2019 6,426 2 View

How can I get better immunohistochemistry results with my lymphatic endothelial cells?

I am attempting to perform some IHCs on Sprague-Dawley rat primary lymphatic endothelial cells, but I can't seem to get consistent results. When I attempt to image my cells after staining, the...

04 April 2019 3,380 1 View