Matthew Whitley

2 Questions 11 Answers 0 Followers

Questions related from Matthew Whitley

Problems with chitin-binding domain fusion protein NOT sticking to chitin resin?

Using the New England Biolabs IMPACT system, we have expressed our protein of interest with a C-terminal fusion of a chitin binding domain (vector is pTXB1 from the NEB IMPACT system). The...

02 October 2019 7,406 3 View

Can anyone tell me how to chemically modify an expressed and purified protein by carbamylating its N-terminus?

Hello all, I am interested in chemically modifying an expressed and purified protein by carbamylating its N-terminus. The difficulty is that most protocols I've found in the literature use 8 M...

16 February 2016 9,918 2 View