Matthew Wengler

4 Questions 5 Answers 0 Followers

Questions related from Matthew Wengler

Detection of soluble recombinant protein aggregates by native PAGE?

I have been able to express a soluble 6xHis-tagged protein but purification is extremely inefficient. We are only able to increase our yield by overloading the resin (8-10 ml lysate on 50 ul...

13 September 2021 5,954 3 View

Could a denatured and refolded his-tagged protein bind resin under native conditions?

I am trying to purify a 10 kDa fungal protein with two predicted disulfide bonds by heterologous expression in E. coli with the aim of performing a pull-down assay to identify host targets. I had...

08 September 2021 7,509 5 View

Cleavage of GST fusion protein during purification?

I am trying to purify a GST fusion protein on a glutathione agarose resin. The fusion protein is expressed in E. coli and an estimated 34 kDa. I have performed a western blot with anti-GST...

19 March 2021 8,323 8 View

Infected plant protein lysate washing out purified GST-tagged protein on agarose resin?

Hi, I am trying to screen infected plant proteomes for targets of a secreted pathogen protein. I have purified the pathogen protein using a GST-tag on an agarose resin. I am using the Pierce...

24 November 2020 7,135 3 View