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Questions related from Matthew Wengler
I have been able to express a soluble 6xHis-tagged protein but purification is extremely inefficient. We are only able to increase our yield by overloading the resin (8-10 ml lysate on 50 ul...
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I am trying to purify a 10 kDa fungal protein with two predicted disulfide bonds by heterologous expression in E. coli with the aim of performing a pull-down assay to identify host targets. I had...
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Hi, I am trying to screen infected plant proteomes for targets of a secreted pathogen protein. I have purified the pathogen protein using a GST-tag on an agarose resin. I am using the Pierce...
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