Matthew J Tarchick

2 Questions 1 Answers 0 Followers

Questions related from Matthew J Tarchick

How many retinal samples are needed for PCR?

I am doing a RT-qPCR of mouse retinas. Each retina is very small. Can I use right and left retinas seperately, or would I need to use both a right and left retina in one mix to get enough sample?

21 November 2014 2,624 2 View

Why are retina fixation procedures inconsistent?

I have been using a procedure for mouse eye fixation in paraformaldehyde. We remove the mouse eye after sacrafice. We immediately put it in 4% pfa for 30 minutes. After that we punch a hole in the...

21 November 2014 558 7 View