Matthew Scarnati

5 Questions 12 Answers 0 Followers

Questions related from Matthew Scarnati

Why am I getting cross reactivity in my Immunohistochemistry experiments?

I am performing IHC using mouse brain slices. I am performing double labeling and the blocking buffer I am using is normal goat serum. One target remains constant in my experiments, and the second...

05 January 2017 5,086 3 View

Why is my double labeling experiment in mouse brain slice resulting in what appears to be cross talk between my antibodies?

I am performing IHC in mouse brain slice. I am using two antibodies for the purpose of co-localization. My first target remains constant in all experiments and is a guinea pig primary. The...

21 December 2016 7,017 3 View

Antibody labeling of synaptosomes?

Does anyone have a good protocol for antibody labeling of synaptosomes?

25 May 2016 821 1 View

How long does it take cycloheximide to inhibit the ribosome, on average?

I was curious if anyone has an idea of the length of time it takes for cycloheximide to inhibit translation. I am working in neurons. Thanks.

17 March 2015 3,680 0 View

Does anyone know the advantages/disadvantages of loading neurons with, FM1-43 versus FM4-64?

I am curious if one is better for certain applications as opposed to the other. Thank you.

30 January 2015 1,162 4 View