Matthew Scarnati

3 Questions 8 Answers 0 Followers

Questions related from Matthew Scarnati

Why am I getting cross reactivity in my Immunohistochemistry experiments?

I am performing IHC using mouse brain slices. I am performing double labeling and the blocking buffer I am using is normal goat serum. One target remains constant in my experiments, and the second...

05 January 2017 5,042 3 View

Why is my double labeling experiment in mouse brain slice resulting in what appears to be cross talk between my antibodies?

I am performing IHC in mouse brain slice. I am using two antibodies for the purpose of co-localization. My first target remains constant in all experiments and is a guinea pig primary. The...

21 December 2016 6,965 3 View

How long does it take cycloheximide to inhibit the ribosome, on average?

I was curious if anyone has an idea of the length of time it takes for cycloheximide to inhibit translation. I am working in neurons. Thanks.

17 March 2015 3,631 0 View