Matthew Koslow

2 Questions 1 Answers 0 Followers

Questions related from Matthew Koslow

Why is my PCR resulting in a 23 BP repeat?

I am trying to sequence a potential KO by submitting samples to Genewiz, and I am getting this repeat in one of my sequences. Here is my PCR Steps -98 degrees for 2 minutes -Touchdown 7 times...

13 August 2020 606 3 View

IDT Alt R Genome Editing Detection Kit Troubleshooting?

Hello, I am have been trying to use the IDT Alt R Genome Editing detection kit to assay some potential clones of KO cells I have generated. This kit is essentially a T7 endonuclease mismatch...

15 May 2020 9,299 0 View