How does culture with anti-CD3/anti-CD28 mAb alone compare to culture with anti-CD3/anti-CD28 mAb and subsequent treatment with PMA/Ionomycine?

Performing FACS analysis on NHP PBMCs cultured ex vivo on anti-CD3/anti-CD28 coated plates for 96 hrs. and then fixed and frozen OR treated with PMA/Ionomycin for 6 hrs prior to fixation and...

30 April 2013 7,389 15 View