Matteo Rovere

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Questions related from Matteo Rovere

Can I and how do I design a bicistronic – two different promoters – lentiviral transfer plasmid (no IRES/P2A)?

Good morning! I am currently cloning some bicistronic expression vectors for immortalized microglia-like cells or other common lines (HeLa, HEK...), with the ultimate goal of generating stable...

22 August 2025 7,764 4 View

How to interpret phosphosite localization using these MS2 data?

Dear all, I have been working on the localization of phosphosites on my protein of interest using a variety of approaches (PhosTag SDS-PAGE, S-to-A mutations etc.) and, among others, I submitted...

14 October 2024 2,019 4 View

Can proteins be co-expressed in E.Coli under the control of two different inducer molecules (i.e. IPTG/arabinose)? Any downsides/known issues?

Hello. In our lab we're trying to express (in BL21(DE3) E.Coli) both the two subunits of the NATB yeast acetylation complex and our target protein (alpha-synuclein) under control of different...

09 February 2016 6,893 7 View