Matteo Marangon

8 Questions 28 Answers 0 Followers

Questions related from Matteo Marangon

What is the best method to measure grapevine leaf area in a non destructive way?

I am looking at different methods to measure/calculate the leaf area of grapevines. I have never calculated the area before and while reading the literature to find the best method I found that...

16 June 2014 8,917 8 View

Problem with standardized extraction of juice from grape samples.

I am looking to buy an instrument for pressing the juice out of grape samples of small size (up to 1 kg of grapes). As the pressure applies to the grape samples can affect the extraction of the...

05 February 2014 8,499 2 View

How to compare the hydropathicity of two similar proteins?

I am trying to compare the hydropathicity of 2 proteins belonging to the same family (about 75% identity). So far I have been using the ProtScale tool in Expasy with the Kyte & Doolittle scale,...

19 November 2013 7,846 8 View

When solving a protein structure, is it preferable to have higher resolution or lower R factors?

I am solving a structure of which I have X-ray data up to 1.5 A resolution. However, there are not so many spots in the highest resolution region, so that when I solve the structure (with a good...

30 July 2013 1,480 54 View

What is the best method for comparing the structural similarity of two proteins?

I have solved the structure of 2 isoforms of the same protein and I have used PyMol and Chimera to superpose them and check for differences. The differences are quite small (RMSD 0.5 A), but I was...

25 July 2013 1,241 28 View

When preparing PDB file for deposition: is there a simple way to add TER cards at the end of a protein chain?

In order to deposit a protein structure on the PDB database the file needs to have a TER card at the end of the chain. In the past I have added them manually in the PDB file and changed the...

17 June 2013 8,562 4 View

Do you know of any proteases active at low pH and low temperatures?

I am trying to degrade globular proteins that are quite resistant to proteolytic enzymes at pH 2.8-3.8 and at temperature below 20ºC. Do you know of any candidate enzyme that I could try? My...

11 June 2013 6,401 13 View

What is the best software to superimpose and compare two isoforms of the same protein?

I have solved the 3D structure of two proteins and I now want to start comparing them to find out analogues residues, regions of similarities/differences etc to ultimately explain the different...

17 April 2013 4,293 9 View