Matteo Cortese

2 Questions 2 Answers 0 Followers

Questions related from Matteo Cortese

How to design an artificial microRNA for RNAi?

I want to silence a gene with a medical purpose in mind. From what I read, the use of shRNA is frowned upon due to toxicity concerns, with researchers turning instead to artificial miRNA for the...

29 September 2021 5,438 0 View

Self-ligation of double digested and dephosphorylated vector?

Hi, This is a brief description of my latest head scratcher: I am digesting a vector with XhoI and HindIII-HF (both from NEB), then I dephosphorylate the digested vector with the Antarctic...

14 August 2021 4,356 3 View