Matt Wiebe

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Questions related from Matt Wiebe

I have been optimizing ChIP with sonication +/- MNase. The MNase IS NEEDED for small DNA fragments, but has NO EFFECT on enrichment. Is this common?

My ChIP protocol with sonication alone gave me good enrichment of my GOI, but made me concerned b/c the DNA fragment sizes were 5+kb at times. Therefore, I added a MNase step (still with...

09 September 2014 8,780 4 View

Different results with rabbit anti FLAG antibody and mouse anti FLAG?

When IPing my FLAG-tagged protein for ChIP I have used rabbit anti-FLAG and mouse anti-FLAG (both from Sigma) The rabbit anti-FLAG is far superior in our hands in regard to enrichment of DNA...

09 September 2014 9,655 2 View