Matt Demelo

4 Questions 3 Answers 0 Followers

Questions related from Matt Demelo

How can I isolate circular single stranded pDNA isoforms?

I'm developing an analytical HILIC-HPLC method, and I am interested in seeing how the oc/ss-pDNA isoforms separate out from other isoforms, and if they do at all. To do this, I need to prepare a...

08 August 2018 7,211 0 View

Are there any good methods for hydrophobic interaction HPLC of plasmid DNA isoforms?

I am trying to develop a method for optimizing separation of different plasmid isoforms in plasmid samples for QC purposes. I was wondering if there was a good method I could test out to try this...

07 July 2018 5,160 2 View

Consequences of using Tris-EDTA buffer instead of Tris-HCl for DNA elution?

I am trying to isolate isoforms of plasmids from agarose gels using a nucleic acid purification kit, and I have been playing around with the my choice elution buffer in order to see if I can...

07 July 2018 5,585 2 View

Discrepancy in OC-pDNA migration in agarose gel?

I recently ran digestions of a plasmid to obtain linearized and open-circular/nicked isoforms through gel extraction. On the first gel (from which I extracted the DNA from, ran this for 2 hours at...

07 July 2018 1,611 6 View