Mats Urban

2 Questions 2 Answers 0 Followers

Questions related from Mats Urban

How do I prevent frozen and thawed Bone Marrow Cells from clumping together?

I need to freeze Mononuclear Bone Marrow cells and thaw them at a later timepoint to analyse them via FACS. To freeze the cells I use 90%FBS and 10%DMSO. But when I thaw the cells (using...

16 May 2022 1,130 5 View

What is the best way to stimulate cells and inhibit protein transport (Monensin vs. Brefeldin A) when Staining for Cytokines (IL-17, IFN-y)?

I am planning on analyzing the cytokine expression of human bone marrow cells via Flow Cytometry. For conducting the intracellular cytokine staining, I am not sure what Protein Transport...

30 March 2022 6,969 1 View