Fixation and permeabilization of spermatozoa (in suspension)

I have a problem between fixation and permeabilization of a sperm sample, 1. After Live/Dead staining, I wash my 100 uL sperm sample (25x106 sperm/mL) with 900 ul of D-PBS and centrifuge for 5...

21 June 2017 3,793 3 View