Mastura Sani

3 Questions 4 Answers 0 Followers

Questions related from Mastura Sani

What happen if I run native protein under denaturing PAGE? Will the size larger than expected?

Hai. I break the cells containing my expressed protein (r-msAdh1) using non - denaturing lysis buffer. Then I run 10 uL lysate under denaturing polyarylamide gel (SDS-PAGE). But I observed that my...

13 March 2020 2,380 9 View

Heterologous expression of eukaryote gene

Hi everyone. Im working with expression of eukaryote gene in bacteria system. I have a question but before that, below is my procedure in the expression. 1. I grow my culture to OD: 06. Upon...

01 January 1970 6,030 3 View

Heterologous expression of eukaryotic gene in E.coli

Salam and Hi. I have a question. Is denatured protein and undenatured protein have the same lenght when view on 12% sds page?

01 January 1970 1,016 4 View