Massimo Caruso

5 Questions 14 Answers 0 Followers

Questions related from Massimo Caruso

Intracellular oxidative stress dosage by Flow Cytometry

Anyone could suggest a good and fast protocol to determine the oxidative stress in cells with dichlorodihydrofluorescein diacetate (DCFH-DA) by FACS?

06 June 2016 8,570 3 View

Is it suitable to stabilise human blood cells by RNA-Later to perform DNA expression studies?

Human blood cells stabilised by RNA-Later to perform DNA expression studies. It is a good way to store white blood cells to perform subsequently the isolation of RNA? Or is it preferable to store...

06 June 2015 2,740 4 View

Does anyone have experience with stimulation of T cells by TNF-alpha and or histamine?

I am trying to activate T cells by stimulating WBC with TNF-alpha and/or histamine. I hope to detect an activation status of CD3 cells detectable by CD69 over-expression (as a model of...

04 April 2015 4,734 9 View

What are the best markers for the identification of Neutrophils, eosinophils and circulating dendritic cells in whole blood by flow cytometry?

I need to study these cells in whole blood samples. I normally use the protocol "Lyse-no-wash". It could be used to study these cells, too?

04 April 2013 3,840 12 View

Do you have any suggestions for commercial kits or technical procedures that can help me perform the TUNEL test on spermatozoa by flow cytometry?

TUNEL test to detect DNA fragmentation on spermatozoa

10 October 2012 9,392 8 View