Mason Sweat

2 Questions 14 Answers 0 Followers

Questions related from Mason Sweat

Why do I see extracellular GFP, with close to no GFP expression in my cells, after transfection?

Hi all, I'm new to eukaryotic transfection. At the moment, I'm trying to transfect a cell line using eGFP as a control. The vector is empty apart from the GFP. After 24 hours, I examine my cells...

02 February 2015 7,468 9 View

Why do I see my protein ladder standard on my developed western blot?

I'm attempting to use a western blot to detect the presence of an endogenous transcription factor. After I have shown that my western conditions are capable of detecting the transcription factor,...

11 November 2014 7,697 10 View