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I ran the same qPCR test (SYBR Green) on a lot of samples that didn't fit on one plate (over 20 plates all together) and want to compare all of them to the same standard curve that was included in...
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The whole volumes of my PCR reactions (10 uL) evaporated during the cycling (honest mistake, I know why it happened and how to fix it - wrong PCR caps used). My question is, could this leave some...
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