Why my transduction efficiency doesn't exceed 10% with MOI=10 in Jurkat cells?

I am expressing two genes (CAR and a reporter) under EF-1a promoter in a lentiviral vector. I am using 2nd-generation lentiviral system (psPAX2 and VSV-G). My transfection efficiency in 293LTV...

25 October 2023 5,750 5 View