Marym Albalwi

4 Questions 6 Answers 0 Followers

Questions related from Marym Albalwi

The protein molecular wight is difference in MS?

Hi, i checked the protein molecular wight by MS, the result is shown higher molecular wight (~1010 Da) than the theoretical one. The protein in 60mM HEPES contains (50 ug/ml peg20000, 3mM MnCl2,...

10 October 2019 9,248 2 View

Protein sample in 100% D2O,pH6?

Hi im doing protein side chain assignment by NMR i want to do 13C-NOESY-HSQC (3D), the result spectrum is used to obtain restraints for structure calculation, normally it will recorded with...

05 May 2018 8,433 0 View

TEV protease in Gel filtration

Hi I used Ni column to purify my his-tagged protein (57 kd), after that, I cleavaged it with TEV protease that gave me 47kd for His-tagged, 52kd un-cleavage proteins and 12 kd my target...

07 July 2017 3,001 6 View

What caused plasmid disappear after checking its concentration by nanodrop ?

Hi After purified my digested plasmid from agarose gel, i used Nano-drop which gave me 33.1ng/ul. After that, I used Gibson to insert my gene and then run PCR 50c for 1/h.I got nothing after...

03 March 2017 5,347 4 View