Is there a possibility of TGA stop codon read-through in BL21pLysS expression host i.e., transformed with pET28a+ construct containing 2 gene operon?

Target bacterial operon is reported to be co-transcribed and co-translated in wild type host. However, protein expression analysis (SDS PAGE) indicates translation of only first gene in frame...

16 April 2021 3,902 3 View