Maryam Khosravian

3 Questions 10 Answers 0 Followers

Questions related from Maryam Khosravian

Any tips for LincRNA amplification?

My RNAseq data and qPCR showed that there is an up-regulation of one lincRNA in the treated cells but when I want to amplify the full length of this lincRNA (1600bp) by RT-PCR, I have no band. Do...

25 April 2022 2,331 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

27 February 2021 10,597 3 View

Transformation into the Stbl3 chemo competent cells?

I am trying to clone a pGreenPuro vector (size 8kb) into stbl3 cells but eventually there is no colony on the plates. I plate them 37 ’C overnight on the LB-agar plates. Any solution?

04 February 2021 1,602 5 View