What is the most sensitive, yet specific method for detecting T-cell responses to tumor antigens when a limited number of PBMCs are available?

Culturing PBMCs with overlapping peptides derived from the sequence of the tumor antigen and IL-2 plus IL-15 prior to analysis by flow cytometry or ELISPOT assay appears to be quite sensitive, but...

07 July 2013 7,330 17 View