Mary Hamilton

3 Questions 12 Answers 0 Followers

Questions related from Mary Hamilton

Why do I get a biphasic curve in an ELISA assay of a protein?

A "biochemist" teaching an Immunology lab. Still trying to learn how to use ELISA results without a KIT! What is the end-point in the sigmoidal semi-log plot of the detection absorbance vs. log...

04 April 2016 8,205 5 View

How to use SNAP.id for processing Western blots using Odyssey's IR detectors?

To save class time, the vacuum system is ideal, but with the ultra-sensitive IR detection (Odyssey) the blots are NOT pretty with blotches sometimes obscuring the protein bands. We use a dilution...

03 March 2016 3,235 1 View

How can one blot proteins from an agarose gel?

I use agarose gel electrophoresis to study enzymes for which I can stain for enzyme activity, but I want to identify the enzymes by specific antibodies on a Western blot. Would "Southern"...

08 August 2015 4,410 6 View