Mary Thomas

2 Questions 6 Answers 0 Followers

Questions related from Mary Thomas

Any advice on why the restriction digestion is not working?

I have fluorescent labelled DNA oligos, having a single BamH1 site. I'm screening some compounds which may affect the enzyme cleavage efficiency. My problem is that the oligo is not getting...

03 November 2015 761 6 View

My protein is not getting purified. How should I proceed with the purification?

I'm expressing a mammalian protein in E.coli. My problem is that although protein is expressing (checked by Western blot), its not binding to the Ni-NTA resin (protein is His-tagged). I tried...

13 May 2015 249 6 View