Mary Ann Pohl

3 Questions 3 Answers 0 Followers

Questions related from Mary Ann Pohl

Advantage of TG1 over other strains in phage display panning?

I have been routinely using TG1 for phage display pannings but am having difficulty obtaining high quality sequence data from clones sequences from phagemid vectors in TG1, likely due to EndA. Is...

04 August 2021 9,282 0 View

Has anyone ever compared phage ELISA to soluble scFv PPE ELISA when screening phage panning output?

I am curious as to whether the difference in amplification signal (due to many copies of pVIII recognized by the anti-M13 antibody vs using a secondary that only recognizes the scFv would result...

27 February 2019 7,683 2 View

Has anyone used magnetic Ni beads for phage display against a His-tagged target protein?

I am wondering how well they would work compared to streptavidin beads and a biotinylated target.

19 August 2016 2,895 4 View