Mary Chuah

2 Questions 5 Answers 0 Followers

Questions related from Mary Chuah

Can anyone explain to me why Direct Bisulfite Sequencing (without cloning ) on Sanger is unrealiable?

Hi all, I am trying to measure differential methylation of CpGs by direct sanger sequencing on Nested PCR products of Bisulfite converted DNA. I've read that most would recommend cloning or...

08 April 2016 4,900 2 View

Anyone experince problems with baseline noise and inconsistent results duringDirect Bisulfite sequencing?

Hi, I am trying to measure the differential methylation of the CpGs in a promoter region of my gene of interest. I have done bisulfite conversion on human genomic DNA extracted from blood...

06 April 2016 3,390 7 View