Marwa Zafarullah

2 Questions 4 Answers 0 Followers

Questions related from Marwa Zafarullah

How can I detect the contamination for qRT PCR in Nuclear and Cytoplasmic Fractions?

Hi!! I am interested in quantifying my gene of interest by qRT PCR and interested in looking for gene who's mRNA expressed either only in Nuclei or cytoplasm to identify the contamination of...

02 November 2018 3,702 2 View

How to generate the cDNA for the amplification of 3kb+ transcript of Antisense gene?

Hi!! I am trying to generate the cDNA by using the Random hexamer and M-MLV RT enzyme for the amplification of the 3kb+ transcript of Antisense gene. The quality of the extracted RNA has been...

07 March 2018 4,574 3 View