Why is the OD values not decreasing using 3 dilution in Indirect ELISA?

I am using indirect ELISA to measure the antibody response against Aeromonas salmonicida in Atlantic salmon. I am using 3 dilutions of serum 1:50, 1:200, and 1:400. The OD450 nm values measured at...

09 February 2021 9,248 3 View