Martino M Gabra

2 Questions 5 Answers 0 Followers

Questions related from Martino M Gabra

Why am I getting a huge PCR band that's not leaving the well?

Summary: So I'm PCRing a 3 kb region off of genomic DNA that's been giving me a really tough time. It wasn't working for a while, but now I got it to work except it's not always there under the...

09 June 2015 7,653 8 View

What is the largest strand of DNA that I can excise and repair using CRISPR/cas9?

I would like to take out about 1kb maybe 3kb of DNA depending on my sgRNA efficiencies, but I would also like to repair the region by inserting another large strand of DNA. In other words, I'm...

13 January 2015 3,294 4 View