Martiniano M Ricardi

3 Questions 36 Answers 0 Followers

Questions related from Martiniano M Ricardi

Has anybody runned GFP (or other fluorescent protein) and detected fluorescence on a semi-native SDS PAGE?

I would like to run a GFP quimeric protein and determine its molecular weight. I need to detect the GFP protein trought Fluorescence. Therefore I can´t just run a regular native gel. Does anybody...

07 July 2015 3,351 2 View

Do proteins in semi native SDS-PAGE run according to MW or to both MW and net charge?

Hi, I have seen several semi-native SDS PAGE protocols. I am wondering if the semin native SDS-PAGE can be used to accurately estimate protein molecular weight? Is the added SDS enought to give...

07 July 2015 7,924 1 View

Can anyone share his/her experience purifying GFP (or GFP variants) in e-coli?

I will soon start expressing GFP-quimeric proteins in E. coli (BL21) and then purifying them using a 6xHIS tag. Does anybody have experience working with recombinant GFP proteins it complicated?...

01 January 2015 3,693 10 View