Martina Tuttolomondo

3 Questions 2 Answers 0 Followers

Questions related from Martina Tuttolomondo

Dimers at western blot but not for all samples?

Hi all, I have repeated this western blot three times. Samples have been incubated with DTT 0.06 M at 99 oC for 5 min. The second time I prolonged the incubation to 10 min. The third time I...

07 July 2018 7,463 3 View

Gene sequencing to check a SNP?

Hello, I have two cells lines: A375 and Mel-ST. This last one is an immortalized cell line from primary melanocytes. The BRAF gene should be mutated with V600E mutation in A375 cells but not in...

06 June 2018 2,543 7 View

Cells detach from the well during fixation. How to stain them?

Hello, I am currently working with a ZR-75-1 cell line that was treated for long time with fulvestrant to acquire resistance. The parental cell line behave normally while I believe that the...

06 June 2018 2,411 2 View