Martin Oheim

3 Questions 5 Answers 0 Followers

Questions related from Martin Oheim

What techniques do you use for calibrating the evanescent-field decay, in vitro and inside cells?

Total internal reflection fluorescence microscopy (TIRFM) permits imaging near-membrane dynamics by selectively exciting fluorophores close to a dielectric interface. However, neither quantifying...

07 July 2014 1,970 0 View

Is anyone aware of a measurement of the fluorescence quantum yield (Ca-free/Ca-bound) of X-rhod1?

In order to calculate the molecular brightness (product of molar extinction coefficient and fluorescence quantum yield) I would like to know that number. Thanks a lot in advance.

01 January 2014 3,307 1 View

Is anyone aware of a quantitative tool that would permit assessing the spectral independence of color channels in mulit-band fluorescence recordings?

(Meaning: how do I know that my two or three or so color channels do really contain independent information?) Thanks.

02 February 2012 669 5 View