Martin Kenny

2 Questions 3 Answers 0 Followers

Questions related from Martin Kenny

BsmbI digestion not working?

I'm using BsmbI from NEB to digest a CRISPR lentiviral plasmid so I can insert my gRNA sequence. The digestion isn't working at all despite extending the reaction time to 1.5 hours. Other answers...

11 November 2017 7,720 1 View

How can I identify a pure neutrophil population in whole blood by flow cytometry?

I want to examine neutrophil populations in whole blood. I've read that dot plots of CD45 and SSC can be used to gate for granulocytes, but I need to exclude basophils and eosinophils from my...

12 December 2016 7,598 7 View