Martin de Boer

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Questions related from Martin de Boer

Does anyone have experience in capturing large fragments of human genomic DNA for Nanopore sequencing?

The biggest challenge in Nanopore sequencing is targeted aproach to select regions of interest from large genomes. Has anyone experience in this field?

04 April 2016 922 2 View

Does anyone have experience with cloning a PCR fragment with HA or V5 tags?

We find only clones in which the HA or V5 tags have been partly removed from the insert sequences. We use PWO polymerase and no hotstart. It looks like the endonuclease activity of the PWO is...

06 June 2014 7,060 1 View