Martijn Vervoort

2 Questions 5 Answers 0 Followers

Questions related from Martijn Vervoort

Why would one dilute human serum in 1% human serum albumin (HSA) in tris-bufferd saline (TBS)?

I'm trying to set-up an new ELISA following the protocol of Vojdani (Antibodies as predictors of complex autoimmune diseases). "Serum samples were diluted 1:200 in 1% HSA in TBS containing 1mg/ml...

11 November 2014 5,880 3 View

Should I use an acid or a base as stop solution at the end of an ELISA?

Does it matter if you use an acid (H2SO4) or a base (NaOH) as stop solution, since the main goal of the stop solution is to end the enzymatic reaction by degrading the...

10 October 2014 9,328 7 View