How to incorporate thymidine analogs by PCR?

I am aiming to create 5 - 10 kb amplicons by PCR with all thymidines replaced by different analogs (i.e. BrdU, EdU, CldU, IdU). For this, I tried to simply replace dTTP by dBrdU (or other) in the...

30 June 2016 9,969 1 View