Martijn den Hoed

2 Questions 2 Answers 0 Followers

Questions related from Martijn den Hoed

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

02 March 2021 9,369 2 View

Buffers for cryo-EM

I'm trying to characterize the 3D structure of the extracellular domain of 300 kDa of a membrane protein. A problem I'm trying to solve is the formation of aggregates when trying to visualize the...

01 January 1970 1,554 2 View