Marta García López

5 Questions 4 Answers 0 Followers

Questions related from Marta García López

It is correct to use GAPDH as housekeeping gene in cells with a mitochondrial disorder?

I understand the importance of choosing a good gene standard for normalization of qPCR values. Even so, I would like to know if it could be performed (unless it is not the best option) the...

07 March 2024 4,622 1 View

How do you denature a membrane protein for Western-blot?

I am working with a protein located in the mitochondrial inner membrane, and I would like to know in which conditions should I perform the denaturation step... maybe the "standard" denaturation at...

03 August 2023 4,152 3 View

Why do I have unspecific CD90 staining in flow cytometry?

I am trying to quantify the percentage of retinal ganglion cells I have in my cell population by surface staining of CD90 using flow cytometry. The protocol I follow have a blocking step with 2%...

03 June 2023 9,123 1 View

Why my retinal ganglion cells derived from iPSCs show a high percentage of apoptosis and reactive oxygen species generation?

I'm trying to measure apoptosis levels and reactive oxygen species generation in retinal ganglion cells generated from induced pluripotent stem cells. For both protocols I use Tryple Express to...

11 May 2023 273 4 View

The mechanism of non-sense mediated decay acts always for nonsense mutations?

I'm wondering if there are any cases in which a nonsense mutation don't trigger non-sense mediated decay. If so, I would like to know if it would be possible to detect truncated protein in a...

01 January 1970 792 1 View