Marta Duque

2 Questions 3 Answers 0 Followers

Questions related from Marta Duque

Sudden appearance of primer-dimers and no PCR amplification, why?

I have been using a pair of primers (designed by my co-supervisor, who is no longer here) to amplify my sequence of interest from chelex extracted genomic DNA. Even though I always had a faint...

10 January 2019 3,852 3 View

Does anyone know of a detailed protocol for adult zebrafish endocrine pancreas dissociation (for FACS)?

I am using a protocol from Tarifeño-Saldivia et al. BMC Biology (2017) to isolate pancreatic beta-cells from adult zebrafish transgenic lines - Tg(Insulin:GFP), however I obtain very low number of...

09 March 2018 9,037 2 View