Marta Barbosa

4 Questions 3 Answers 0 Followers

Questions related from Marta Barbosa

Anyone made a puromycin curve for the cell lines MC38?

Hi! Anyone made a puromycin curve for the cell line MC38? Thanks

11 August 2025 3,247 0 View

Anyone made a puromycin curve for the cells lines Hepa1-6, MC38, SW948 and LS174T?

Hi everyone! Does anyone made a puromycin curve for the cells lines Hepa1-6, MC38, SW948 and LS174T where was observed 100% cell death on days 5 and 7? Thanks in advance, Kind regards

04 August 2025 6,178 0 View

My cells are dying after treatment puromycin?

Hi. I'm establishing the cell lines SW948 and LS174T with lentivirus that carry the gene with puromycin resistance (no fluorescence tag). I performed the transduction with polybrene and selected...

29 July 2025 3,312 2 View

Amplification in "No RT" sample during RT-qPCR?

Hi everyone, I am analysing the gene expression from different Drosphila genes by RT-qPCR. I am having problems with one of the gene, OR24a, because I see amplification in the "No RT" sample,...

27 December 2023 8,620 4 View