Mars Yang

4 Questions 4 Answers 0 Followers

Questions related from Mars Yang

Trouble recovering peptides after iodoTMT enrichment - Why No Detectable Peptide?

Dear colleagues, I have been testing an iodoTMT-based workflow, but after enrichment I am unable to detect any peptide. A brief summary of my protocol is below: IAA alkylation of free thiols...

07 July 2025 6,297 1 View

Fluorescence signal higher with iodoacetamide (IAA) blocking (redox proteomic) - any insights?

Hi all, I am currently working on an experiment to determine the optimal concentration of iodoacetamide (IAA) for blocking cysteine (Cys) thiol groups in protein samples for our redox proteomic....

10 September 2024 8,117 2 View

Using BODIPY™ TMR C5-Maleimide to detect free thiols in protein samples: Which method is better?

We plan to use BODIPY™ TMR C5-Maleimide to detect free thiols in our protein samples and have designed two approaches. We would appreciate any advice on which method might be more suitable. 1....

21 August 2024 4,386 2 View

Any tips for cell counting in 96-well plate?

Dear colleagues, I am currently using HCT-116 adherent cells for cell culture in 96-well plates and have some questions regarding cell counting. I have tried various methods and found the most...

23 June 2024 1,256 5 View