Marlies Spaans

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Questions related from Marlies Spaans

Is the Phire Hot Start II DNA Polymerase suitable for reverse transcriptase-PCR?

I'm doing a RT-PCR on orchids, but the program I use now takes up to 3 hours. It would be nice if I could reduce that time with Phire. However, I'm not sure if it affects the gene-expression. Are...

06 June 2019 5,697 5 View

What staining is best to detect lignin in angiosperm stems embedded in LR-white?

I'm trying to determine the grade of lignification in the peduncles of different angiosperms using ImageJ. First I embedded the stems in paraffin and stained them with Etzold's solution (beautiful...

04 April 2019 5,646 2 View

What is the best way to store RNA isolated using the Qiagen RNeasy Plant Mini Kit?

For my thesis I am isolating RNA from Fusarium infected tulip bulbs to study a certain gen expression. I am using the Qiagen RNeasy Plant Mini Kit for isolating the RNA. The kit comes with RNase...

11 November 2017 2,337 2 View