Ligation of concatamers failed, why?

I want to clone 3-6 repeats of a 100bp annealed oligo (AO) into a vector. After hybridization of the AO I see distinct bands of 100, 200, 300, 400, 500, 600 and 700 bp on a agarose gel. I cut...

27 August 2018 7,505 3 View