Marko Vukovic

2 Questions 1 Answers 0 Followers

Questions related from Marko Vukovic

Can I insert a TEV cleavage site using site directed mutagenesis kit?

I am using a construct that does not have a TEV cleavage site between my protein and the 6xHIS tag, and I was wondering if I could use Q5 site directed mutagenesis kit to insert it or would I need...

17 April 2022 9,043 4 View

If you have a membrane protein solubilized using 0.033% DDM, how feasible it is to obtain usable SAXS data?

I'm aware that the detergent signal will be very strong and might dominate the spectrum and I have no access to facilities to collect SANS data which would solve the issue.No other detergents...

06 February 2021 6,077 0 View