Can I do a His-Tag (Ni-NTA) purification without using columns? Only my mixing and washing with centrifuge?

My question is simple: How much worse is it to do IMAC puricifation without a column? My plan is to just mix the resin with and E.Coli lysate, wash and elute it with centrifugation steps. I...

07 May 2021 3,714 5 View